To remove the regulator, make sure to close the cylinder valve by turning it to the right. Then you must release pressure from the inflator, by depressing the nozzle as if you are filling a balloon. You will hear some gas release. If the gas release does not stop quickly; you forgot to close the cylinder. You can then unscrew the hand tight connection, and remove the inflator from the cylinder. Never transport a cylinder with a nozzle attached.